Select this product for Molecular Innovations name, Rat tPA Total Antigen ELISA Kit and product number, RTPAKT-TOT. This rat tissue-type plasminogen activator (tPA) total antigen assay is intended for the quantitative determination of total tPA in rat plasma and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. Rat tPA will bind to the affinity purified capture antibody coated on the microtiter plate. Free and complexed tPA will bind to the plate. After appropriate washing steps, monoclonal anti-rat tPA primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the peroxidase conjugated secondary antibody. Following an additional washing step, TMB is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of rat tPA. Color development is proportional to the concentration of tPA in the samples. More Details: Detection Target: tPA Reactivity: Rat Range: 0.1-50 ng/ml Sensitivity: 0.012 ng/mL At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results. Please note ŌĆō this product replaces our old product number IRTPAKT-TOT (same product, new number).