Bovine Ubiquitin ELISA Kit from Innovative Research is intended for the quantitative determination of Bovine Ubiquitin in biofluid samples, such as serum, plasma and other biological fluids. This is a sandwich ELISA in strip well format with reagents for up to 96 tests . Ub present in samples reacts with an anti-Bovine Ub capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-Ub detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active Ub in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Bovine Ub. Detection Target: Bovine Ubiquitin Reactivity: Bovine Range: 0.156-10ng/mL Sensitivity: 0.059ng/mL Storage Conditions: Store all components at 4┬░C This Bovine Ub activity assay is for the quantitative determination of Ubiquitin in samples including serum, plasma and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.