Bovine Tumor Necrosis Factor Alpha ELISA Kit from Innovative Research is intended for the quantitative determination of Bovine Tumor Necrosis Factor Alpha in biofluid samples, such as serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. This is a sandwich ELISA in strip well format with reagents for up to 96 tests . TNFa present in samples reacts with an anti-Bovine TNFa capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-TNFa detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active TNFa in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Bovine TNFa. Detection Target: Bovine Tumor Necrosis Factor Alpha Reactivity: Bovine Range: 78.1-5000pg/mL Sensitivity: 32pg/mL Storage Conditions: Store all components at 4┬░C This Bovine TNFa activity assay is for the quantitative determination of Tumor Necrosis Factor Alpha in samples including serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.