Bovine Creatine Kinase Muscle ELISA Kit from Innovative Research is intended for the quantitative determination of Bovine Creatine Kinase, Muscle in biofluid samples, such as serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. This is a sandwich ELISA in strip well format with reagents for up to 96 tests . CKM present in samples reacts with an anti-Bovine CKM capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-CKM detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active CKM in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Bovine CKM. Detection Target: Bovine Creatine Kinase, Muscle Reactivity: Bovine Range: 12.5-800U/L Sensitivity: 4.65U/L Storage Conditions: Store all components at 4┬░C This Bovine CKM activity assay is for the quantitative determination of Creatine Kinase, Muscle in samples including serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.